High definition wholesale Magneti neodim for Suriname Factory
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Laminated Glass with EVA Film
Laminated Glass with EVA Film is an important kind of laminated glass in glass industry .The processing principle of EVA laminated glass is similar to most of the laminated glass . Furthermore, using EVA film to laminating glass does not require autoclave. All laminating processing will be finished and completed in one vacuum laminator. EVA Film for laminated glass has excellent transparency, outstanding adhesion and aging-resistance (more than 15 years), can be used for architectural glass, decorative glass with fabric, privacy glass, auto glass etc.
Names of EVA FILM for Laminated Glass
Some will call EVA Film in different name:
-laminated glass eva interlayer
-ethylene vinyl acetate film
-solar eva film
-glass lamination film
-glass laminating film
-laminating film rolls
-safety glass film
-tempered glass film
-security glass film
-glass eva film
-security laminate film
-decorative glass film
-stained glass film
EVA stands for ethylene vinyl acetate, a copolymer resin used in the production of laminated glass as the interlayer sandwiched between two pieces of glass. The main function of the interlayer is to stick the two piece of glass together, in order to make the sandwiched glass safer and more secure.
EVA Film is made out of macromolecule material and it performs excellent in bonding strength, heat resistance, cold resistance, humidity resistance and tensile strength in laminated glass. It has been partly taking the places of PVB film in laminated safety glass, laminated art glass, laminated color glass.
This PCR lecture explains about different types of PCR like nested PCR, realtime PCR, quantitative PCR, multiplex PCR, hot start PCR. It explains the principle of polymerase chain reaction techniques in details.
Download the study materials here-
Assembly PCR or Polymerase Cycling Assembly (PCA): artificial synthesis of long DNA sequences by performing PCR on a pool of long oligonucleotides with short overlapping segments. The oligonucleotides alternate between sense and antisense directions, and the overlapping segments determine the order of the PCR fragments, thereby selectively producing the final long DNA product.
Asymmetric PCR: preferentially amplifies one DNA strand in a double-stranded DNA template. It is used in sequencing and hybridization probing where amplification of only one of the two complementary strands is required. PCR is carried out as usual, but with a great excess of the primer for the strand targeted for amplification. Because of the slow (arithmetic) amplification later in the reaction after the limiting primer has been used up, extra cycles of PCR are required. A recent modification on this process, known as Linear-After-The-Exponential-PCR (LATE-PCR), uses a limiting primer with a higher melting temperature (Tm) than the excess primer to maintain reaction efficiency as the limiting primer concentration decreases mid-reaction.
Dial-out PCR: a highly parallel method for retrieving accurate DNA molecules for gene synthesis. A complex library of DNA molecules is modified with unique flanking tags before massively parallel sequencing. Tag-directed primers then enable the retrieval of molecules with desired sequences by PCR.
Helicase-dependent amplification: similar to traditional PCR, but uses a constant temperature rather than cycling through denaturation and annealing/extension cycles. DNA helicase, an enzyme that unwinds DNA, is used in place of thermal denaturation.
Hot start PCR: a technique that reduces non-specific amplification during the initial set up stages of the PCR. It may be performed manually by heating the reaction components to the denaturation temperature (e.g., 95°C) before adding the polymerase. Specialized enzyme systems have been developed that inhibit the polymerase’s activity at ambient temperature, either by the binding of an antibody or by the presence of covalently bound inhibitors that dissociate only after a high-temperature activation step. Hot-start/cold-finish PCR is achieved with new hybrid polymerases that are inactive at ambient temperature and are instantly activated at elongation temperature.
Intersequence-specific PCR (ISSR): a PCR method for DNA fingerprinting that amplifies regions between simple sequence repeats to produce a unique fingerprint of amplified fragment lengths.
Inverse PCR: is commonly used to identify the flanking sequences around genomic inserts. It involves a series of DNA digestions and self ligation, resulting in known sequences at either end of the unknown sequence.
Multiplex-PCR: consists of multiple primer sets within a single PCR mixture to produce amplicons of varying sizes that are specific to different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test-run that otherwise would require several times the reagents and more time to perform. Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes. That is, their base pair length should be different enough to form distinct bands when visualized by gel electrophoresis.
Nested PCR: increases the specificity of DNA amplification, by reducing background due to non-specific amplification of DNA. Two sets of primers are used in two successive PCRs. In the first reaction, one pair of primers is used to generate DNA products, which besides the intended target, may still consist of non-specifically amplified DNA fragments. The product(s) are then used in a second PCR with a set of primers whose binding sites are completely or partially different from and located 3′ of each of the primers used in the first reaction. Nested PCR is often more successful in specifically amplifying long DNA fragments than conventional PCR, but it requires more detailed knowledge of the target sequences.
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